The human osteocalcin promoter directs bone-specific vitamin D-regulatable gene expression in transgenic mice.
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GFAP promoter directs astrocyte-specific expression in transgenic mice.
Glial fibrillary acidic protein (GFAP) is an intermediate-filament protein expressed abundantly and almost exclusively in astrocytes of the CNS. We are studying transcriptional regulation of the GFAP gene to gain insight into astrocyte function and also to develop an astrocyte-specific expression system for manipulating brain physiology. In this work, we have produced transgenic mice carrying t...
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BACKGROUND Uromodulin is the most abundant protein found in the urine of mammals. In an effort to utilize the uromodulin promoter in order to target recombinant proteins in the urine of transgenic animals we have cloned a goat uromodulin gene promoter fragment (GUM promoter) and used it to drive expression of GFP in the kidney of transgenic mice. RESULTS The GUM-GFP cassette was constructed a...
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Genomic DNA from the mouse pulmonary surfactant protein C (SP-C) gene was analyzed in transgenic mice to identify DNA essential for alveolar type II cell-specific expression. SP-C promoter constructs extending either 13 or 4.8 kb upstream of the transcription start site directed lung-specific expression of the bacterial chloramphenicol acetyl transferase (CAT) reporter gene. In situ hybridizati...
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ژورنال
عنوان ژورنال: Molecular Endocrinology
سال: 1993
ISSN: 0888-8809,1944-9917
DOI: 10.1210/mend.7.3.8483481